An ABI 3000 triple quadrupole mass spectrometer was graciously donated by Merck to the Proteomics Facility. This instrument will fill a gap created when the Thermo Finnigan TSQ 7000 was retired in 2008. A triple quadrupole LC-MS/MS system is essential to many researchers requiring biological small molecule MS and MS/MS analyses. Often these types of analyses require upfront HPLC separations. The Department of Chemistry does not offer LC separations and does have a triple quadrupole mass spectrometer. The AZCC only offers this service to members of the AZCC and has 1-2 months backlog of work. ALEC is the only other resource but is strategically designed to analyze environmental small molecule contaminants from soil, water, etc. The Proteomics Core of the SWEHSC used to operate up until 2008, a TSQ 7000 LC-MS/MS system. It has retired this 15 yr-old instrument due to no vendor support and lack of replacement parts, and only offers limited biological small molecule LC-MS/MS work on a 4000 QTRAP LC-MS/MS system. This instrument (4000 QTRAP) is heavily used for quantification of peptides, does not have an APCI source (often needed for small molecules for efficient ionization), and requires extensive downtime to switch from peptide to small molecule analysis. The donated ABI3000 triple quadrupole mass spectrometer from Merck represents a renewed opportunity to have a dedicated triple quadrupole mass spectrometer for biological small molecule analysis from complex biological matrices (urine, plasma, blood, etc.). The ABI3000 will join the fleet of MS instruments in the Proteomics Service Facility in the BIO5 Keating building (room 106).