Deconvolution microscopy

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Deconvolution fluorescence microscope facility

Location: Medical Research Building 330M (see map)
Contact: Dr. David Elliott, 626-7870
Administration: COM Research Microscopy Core Service
Fees for Services: see below 

Don’t mix immersion oils from different vendors

Be careful when reviewing your samples at high magnification on two different brands of microscope.  If you find that you need immersion oil to see fine details in your sample, please do not “mix” two different brands of immersion oil.  Vendors tell us that they have seen instances where the two different synthetic oils combine on the lens surface and form a sticky substance that&rsq

Light microscope objective lenses – Magnification is important, but so are NA and WD.

Many of us have looked though the eyepiece of a department store microscope and seen a fuzzy looking “something” with the highest magnification objective lens.  It’s not completely surprising that an inexpensive lens would give a blurry image.  There are many optical aberrations that need to be corrected to manufacture the expensive lenses that are used on research grade micros

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