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Overview of University facilities & cores
By presenting these microscopy and/or imaging related facilities in a tabular format, we hope to make it easier for users to see what is available on the University of Arizona campus. The links below go directly to the facility's home page, if one exists.
Facilities marked with *** are considered part of the Cellular Imaging Shared Service.
| Tissue preparation & staining for light microscopy | |||
| Histology Service Lab *** | Andrea Grantham (626-4415) |
AHSC 4212 | Routine paraffin (embedding, sectioning & staining) and frozen section (sectioning & staining) histology and special stains. |
| Tissue Acquisition & Cellular/Molecular Analysis Shared Service (TACMASS) | Kathy McDaniel (626-2448) |
AZCC 3959 | Routine paraffin and frozen section histology. Tissue microarrays. Automated immunohistochemical staining with over 80 human and mouse antibodies. |
| DMetrix microscope slide scanner | Lynne Richter (626-2796) |
AHSC 5210 | Can scan an entire 1x3 glass microscope slide in color at 20x. Exports digital images of the scans in a variety of file formats and magnifications. |
Microscope with color CCD camera for routine light microscopy imaging?
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There is a microscope like this at the University Spectroscopy & Imaging Facilities on the main campus (see below). There is also a lower resolution color camera (710x512) on an image analysis workstation in the AZCC (no support contact). For greyscale images, see the CBA Image Acquisition & Analysis workstation (below). Individual labs and departments may have this type of equipment, ask around. *** SWEHSC investigators should contact Mr. Cromey for information about a microscope like this that is available through the generosity of one of the Center's labs. |
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| Fluorescence-related | |||
| ARL Confocal Microscopy facility *** | Doug Cromey (626-2824) |
LSN 410 | Zeiss LSM 510Meta-NLO multiphoton confocal microscope. |
| Cancer Imaging Shared service | Carl Boswell (954-7053) |
AZCC 0953 | Nikon PCM2000 confocal, 3 color imaging of fixed samples. |
| Cytometry Shared Service *** | Paula Campbell (621-2047) |
AZCC | Full service flow cytometry and cell sorting. If you are interested in quantitating fluorescence intensity, flow is the way to go. |
| Intravital microscope | Urs Utzinger | BIO5 | Currently being built in BME. Microscope stage will accommodate small animals for microscopic imaging of organs and tissues. Confocal and multiphoton, as well as fluorescence lifetime imaging. Expected to be ready in 2009. |
| In Vivo Bioluminescence | Brenda Bagget | MRB 130 & BRL | Whole animal imaging using internal bioluminescent probes and high-sensitivity camera. Part of SWAIR (see below). |
| Research Microscopy Core Service (Medicine) *** | David Elliott (626-7870) | MRB 330M | Deconvolution fluorescence microscope. Ideal for low-light samples. Has capability for time-lapse imaging of live cells. |
| Electron Microscopy, AFM and SPM | |||
| AHSC Imaging Facility *** | Tony Day (626-6009) |
LSN 410 | Biological transmission electron microscopy. Tissue processing, sectioning and microscope operation. |
| Keck Center for Nanoscale Characterization | Paul Lee | Chemical Sci 135 | Atomic force and Scanning probe microscopes. |
| University Spectroscopy & Imaging Facilities *** | David Bentley (biological) Gary Chandler (materials) |
Marley 101 | Transmission & Scanning electron microscopy facility, both biological & materials sciences expertise are available. |
| Computerized Image Analysis | |||
| CBA Image Acquisition & Analysis workstation *** | Doug Cromey (626-2824) | LSN 429 | Inverted microscope with multiple imaging modalities and 10bit greyscale CCD camera. Can perform time-lapse imaging on live cells with this instrument. SimplePCI Image analysis software can be used for counting, measuring, and analyzing images from any source. Users perform their own analysis with assistance from Doug Cromey in setting up the semi-automated software macros. Note, identical image analysis software exists on a workstation in the AZCC, however, there is no designated support contact to assist users. |
| Scientific Visualization Lab (UITS) | Marvin Landis | Campus Computer Center | 3D reconstruction and visualization. Immersive 3D environment. |
| Radiology imaging | |||
| Southwest Animal Imaging Resource (SWAIR) | See website for contacts | Part of ARIBI. Includes Single Photon Emission Computed Tomography (SPECT), Biological Magnetic Resonance (MRI), Optical Coherence Tomography (OCT), and in vivo bioluminescence (see above) | |
