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CELLULAR IMAGING FACILITY CORE |
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Cellular Imaging - Home
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Handouts
About these Written Materials
Our handouts are written with local users from the Southwest Environmental Health Sciences Center (or the Arizona Health Sciences Center) in mind. Some of these handouts originally appeared in this Facility Core's regular (5x per year) newsletter.
Some of the material here will be useful to others from outside the University of Arizona who may have similar equipment. We cannot answer specific support questions from outside the SWEHSC. Please be aware that your instrument may have slightly different software or hardware than ours and that the rules for what users are allowed to do on any specific piece of equipment vary widely.
Fair use:
Individuals are welcome to print copies of these handouts for their personal use. Any other use of these materials (classroom materials, core facility distribution, etc) requires permission (contact information is available here).
You are welcome to link to these materials from other sites on the WWW, however, we specifically request that you not host these files on your own servers. We have tried to keep the URLs consistent so that we don't "break" outside links. We do update these files periodically, so the most current version will only be available on this web server.
We are a grant-funded center and we would like to demonstrate to our funding agency (using website logs and emailed requests for specific use as class materials, etc) that the materials available here are of value to the greater scientific community. |
Tutorials and General Information
These handouts are tutorials on specific topics or provide information of a more general nature. These handouts should be of interest to anyone interested in microscopy or digital imaging.
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General |
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Basic Microscope Alignment - Mar 2002
An introduction to aligning a light microscope for Koeller illumination. (2 pages) |
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Light Microscopy Tips - Jul 2004, minor revision Oct 2004
A collection of tips, including information about: Recommended coverslip thickness, Coverslip mounting media, Don’t mix immersion oils from different vendors, Mixing and matching microscope objectives, and Cleaning Microscope Objectives. (4 pages) |
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Formaldehyde Fixatives - 1994, revised Apr 2004
Background information on formaldehyde, optimal fixation suggestions and safety information. (3 pages)
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Digital Imaging |
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Digital Imaging Ethics - Feb 2001, significantly revised in Sept 2004 and May 2007, updated Jun 2010
An overview of the issues related to digital image ethics. Includes a list of twelve guidelines for editing digital images in an ethical and scientific manner. (6 pages) |
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Potentially the most dangerous dialog box in Adobe Photoshop™ - Feb 2002
Tips and warnings about the Image Size dialog box in Photoshop™. (1 page) |
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Using the Histogram Tool - Jul 2000
Understanding this often overlooked tool that is found in most image analysis/editing programs. (1 page) |
Instrument or University of Arizona-specific Handouts
These handouts are specific to one of the instruments available in this Facility Core or contain information that is geared toward University of Arizona affiliated persons.
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General |
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Contact information for affiliated imaging facilities - updated Jun 2010
Names, phone, www, and email information for the people responsible for the major pieces of equipment/service labs affiliated with the SWEHSC Cellular Imaging Facility Core. (1 page) |
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Light Microscope Vendors for Southern Arizona - updated April 2011
Names and contact information for the local service and sales representatives for Leica, Nikon, Olympus and Zeiss. In addition, two local firms (UA Stores vendors) for microscope service and repair are featured. (1 page) |
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Compix Image Analysis Workstations |
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Color Image Capture - AZCC Image Analysis Workstation - Oct 22, 2004
Instructions for capturing color digital images using the attached brightfield microscope. (5 pages) |
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Zeiss LSM 510NLO-Meta |
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Installing the Zeiss LSM Image Browser Software - updated Sept 2005
Instructions for installing this free software on a Windows™ PC. (2 pages) |
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Zeiss LSM Image Browser Software - Sept 2002
Instructions on how to use this software. Important information about exporting files from the proprietary LSM format to other image file formats. (6 pages) |
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There are 5 things that can significantly affect the quality of images on the Zeiss 510 - Sept 2008
Tips on adjusting the detector gain, amplifier offset, pinhole size, scan speed and laser power to obtain better looking images on the Zeiss LSM 510. (3 pages) |
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INFORMATION
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