Microscopy and Imaging banner


On this page:  Technique  |  Immuno/Histo/Cyto-chemistry  |  Internet Resources  |  Histology Images  |  Safety


General Histo-technique


Histotechnology Tutorials
If you are unfamiliar with the practice of histotechnology, this is an excellent place to start.  This series of pages includes a tutorial on general Histotechnique (fixation, tissue processing, sectioning, general staining and slide making information), Special Stains (listed by what types of molecules/tissues that they stain) and examples from their Procedure manuals (note: you will need the free Adobe Acrobat reader to view the procedure manuals).  (WebPath, University of Utah, College of Medicine)
Histopathology Laboratory Protocols
This site has a wealth of technical information about histologic technique.(The University of Nottingham and the Queens Medical Centre, United Kingdom).

Histologic Special Stains Using a Microwave
This page written by Joyce Moore, of the Jefferson Regional Medical Center Histo-Path Laboratories, outlines the use of Microwaves in histologic stainingOther interesting pages at this site (WWW site courtesy of EBSciences)Microwave Antigen Retrieval Technique, Working Procedures in Microwave Histology and Microwave Processing Techniques for Microscopy & Histology.
Laboratory Protocols
An excellent resource with information about: cryostat sectioning, histologic fixatives, histologic stains, peroxidase techniques, treating slides for "stickiness", tissue processing and specimen collection (Veterinary Pathology, University of Bristol, UK).
Protocols On-Line - Immunology>Histological Techniques
StainsFile: A Resource for Histotechnologists
This site, developed by Bryan D. Llewellyn, is primarily about histologic staining techniques and dyes.
The Histology Page
Includes extracts from Woods and Ellis, "Laboratory Histopathology: A Complete Reference" (1994 Churchill Livingstone). Topics include: Cryotechniques, Fixation, Haematoxylin and Counterstains, Microtomy, Mountants, Resin Processing and Tissue Processing.
Tissue Preparation for Histology:
The WWW pages Use and Misuse of Formaldehyde Fixatives and Bouins Fixatives were developed for this site in response to some local concerns.




One resource is the Immunohistochemistry page of Roy Ellis, which includes suggestions for control tissues for different stains and a sample protocol that is in an ISO 9000 compliant format.
Journal of Histochemistry & Cytochemistry
"The official Journal of the Histochemical Society, publishes papers that report the development of new histochemical and cytochemical methods, significant modifications of existing techniques, or original research in which histochemical and cytochemical methods make a significant contribution." Many excellent full-text articles are available on-line.
Fluorescence Techniques
A web resource from this site with information about fluorescent dyes, antibodies and sample preparation.
Miscellaneous Protocol Resource Sites
NIEHS Laboratory of Experimental Pathology - Immunohistochemistry protocols
ImmunoPortal.com - Immunohistochemistry & In-Situ Hybridization (Hogne Røed Nilsen) may be a dead link (checked 10/29/13)


Internet Resources


HistoNet listserver
Scroll down this page to read the description of this email discussion forum for histotechnique (MicroWorld Resources). Visit this site to search the Archives of the HistoNet Listserver.
Histology Glossary
An on-line glossary of terms used to describe histologically prepared specimens (Dr. Mark Hill).
Anatomy & Histology Center - Martindale's Health Science Guide
A collaborative effort of Jim Martindale and the University of California, Irvine (among others). This is an incredible compilation of links to histology & anatomy resources, as well as other biological links and some terrific general science reference material links.

The Internet Pathology Laboratory for Medical Education (University of Utah, College of Medicine)


Images of Tissues prepared for Histology


LUMEN: Histology Index
This site is part of the Loyola University Medical Education Network (LUMEN).
Kansas "JayDoc" HistoWeb
From the Department of Anatomy & Cell Biology, University of Kansas.
University of Delaware - Mammalian Histology course
Includes images from histologic sections, electron microscopy, diagrams and 3D models.

Other Good General Histology Sites with Images:

University of Michigan - Histology course
University of Western Australia - Histology & Human Anatomy
University of Illinois - Internet Atlas of Histology
University of Arkansas - Microanatomy Web Atlas (Gwen Childs)
University of Pennsylvania - Veterinary Histology
Good sites for Images of specific tissues:
"The Whole Brain Atlas" - Harvard University




Repetitive Strain Disorders
Some general background information on this problem (PDF - Leica Microsystems Nussloch GmbH).
The Vermont SIRI MSDS archive
If you can't locate the Material Safety Data Sheet that came with a particular product, this is a good place to start. Another resource can be found at MSDSsearch.com.
Occupational Safety & Health Administration (OSHA, USA):
Laboratory Safety, Bloodborne Pathogens, Ionizing Radiation, Ergonomics



top of the page link graphic