Translational and Clinical Researchers

John Clarke, Ph.D.

Dr. John Clarke is currently a postdoctoral fellow in the Pharmacology and Toxicology Department at the University of Arizona in Tucson Arizona and a member of SWEHSC RFG3.  He received his BS in biology from Brigham Young University-Idaho (2005) and his PhD in Molecular and Cellular Biology from Oregon State University (2011).  Dr. Clarke’s current research interests in the laboratory of Dr. Nathan Cherrington involve investigating how altered xenobiotic transporter expression in liver diseases can 1) contribute to the occurrence of adverse drug reactions and 2) alter exposures to and toxicities from toxic substances.  Dr. Clarke’s professional goal is to continue investigating xenobiotic toxicities as a member of the faculty at an academic research institution.

Dr. Clarke has taken full advantage of the NIEHS training opportunities available to him in order to advance his career as an NIEHS scientist. These opportunities include gaining appointment to the NIEHS training grant, participation in SWEHSC events, obtaining funding through the SWEHSC (pilot project and career development awards), and submitting applications for an NIEHS pathway to independence grant (K99/R00).  He has been actively engaged in the Society of Toxicology (SOT) as postdoctoral representative to the Mechanisms Specialty Section, as a Counselor for the SOT Postdoctoral Assembly Executive Board, winner of the Gabriel L. Plaa Education Award, and winner of the Best Postdoctoral Publication Award. 

Recent publications:

Clarke J.D., Cherrington N.J. Genetics or environment in drug transport: the case of organic anion transporting polypeptides and adverse drug reactions. Expert Opin Drug Metab Toxicol 8:349-60; 2012. PMID 22280100

Meyer M., Kesic M.J., Clarke J.D., Ho E., Simmen R., Diaz-Sanchez D., Noah T.L., Jaspers I. Sulforaphane induces SLPI secretion in nasal mucosa. Resp Med. 107:472-75; 2013. PMID 23195333

Clarke, J.D., Novak, P., Lake, A.D., Shipkova, P., Aranibar, N., Robertson, D., Reily, M.D., Lehman-McKeeman, L.D., Cherrington, N.J. Characterization of hepatocellular carcinoma related genes and metabolites in human non-alcoholic fatty liver disease. Dig Dis Sci: 59:365-74; 2014. PMID: 24048683

Clarke, J.D., Sharapova, T., Maher, J.M., Blomme, R., Cherrington, N.J. Circulating microRNA 122 as a Biomarker of Liver Injury in a Mouse Model of Non-alcoholic Steatohepatitis. J Appl Toxicol: 34:726-32; 2014. PMID: 24217942 

Merrell, M.D., Nyagode, B.A., Clarke, J.D., Cherrington, N.J. Morgan, E.T. Selective and cytokine-dependent regulation of hepatic transporters and bile acid homeostasis during infectious colitis in mice. Drug Metab Dispos: 42:596-602; 2014. PMID: 24378326

Canet, M.J., Hardwick, R.N., Lake A.D., Dzierlenga, A.L., Clarke, J.D., Cherrington, N.J. Modeling human nonalcoholic steatohepatitis-associated changes in drug transporter expression using experimental rodent models. Drug Metab Dispos: 42:586-95; 2014. PMID: 24384915

Clarke, J.D., Hardwick, R.H., Lake, A.D., Canet, M.J., Cherrington, N.J. Experimental nonalcoholic steatohepatitis increases exposure to simvastatin hydroxy acid by decreasing hepatic organic anion transporting polypeptide expression. J. Pharmacol Exp Ther: 348:452-8; 2014. PMID: 24403518

Clarke, J.D, Hardwick, R.H., Lake, A.D., Lickteig, A.J., Goedken, M.J., Klaassen, C.D., Cherrington, N.J. Synergistic Interaction between Genetics and Disease on Pravastatin Disposition. J. Hepatol. 61:139-47; 2014. PMID: 24613363.   *Selected for a focus piece in Journal*

Hardwick, R.N., Clarke, J.D., Lake, A.D., Canet, M.J., Anumol, T., Street, S.M., Merrell, M.D., Goedken, M.J., Snyder, S.A., Cherrington, N.J. Increased susceptibility to methotrexate-induced toxicity in non-alcoholic steatohepatitis. Tox. Sci. 142:45-55; 2014. PMID: 25080921

Canet, M.J., Hardwick, R.N., Lake, A.D., Dzierlenga, A.L., Clarke, J.D., Goedken, M.J., Cherrington, N.J. Renal Xenobiotic Transporter Expression is Altered in Multiple Experimental Models of Nonalcoholic Steatohepatitis. Drug Metab Dispos: 43:266-72; 2015. PMID: 25488932

Dzierlenga, A.L., Clarke, J.D., Hargraves, T.L., Ainslie, G.R., Vanderah, T.W., Paine, M.F., Cherrington, N.J. Mechanistic Basis of Altered Morphine Disposition in Nonalcoholic Steatohepatitis. J. Pharmacol. Exp. Ther. Epub ahead of print. PMID: 25512370

Clarke, J.D., Cherrington, N.J. Nonalcoholic Steatohepatitis in Precision Medicine: Unraveling the Factors that Contribute to Individual Variability. Pharmacol Ther. Accepted