Histology

General Histo-technique

Histotechnology Tutorials

If you are unfamiliar with the practice of histotechnology, this is an excellent place to start.  This series of pages includes a tutorial on general Histotechnique (fixation, tissue processing, sectioning, general staining and slide making information), Special Stains (listed by what types of molecules/tissues that they stain) and examples from their Procedure manuals (note: you will need the free Adobe Acrobat reader to view the procedure manuals).  (WebPath, University of Utah, College of Medicine)

Histopathology Laboratory Protocols

This site has a wealth of technical information about histologic technique.(The University of Nottingham and the Queens Medical Centre, United Kingdom).

Histologic Special Stains Using a Microwave

This page written by Joyce Moore, of the Jefferson Regional Medical Center Histo-Path Laboratories, outlines the use of Microwaves in histologic staining.  Other interesting pages at this site (WWW site courtesy of EBSciences):  Microwave Antigen Retrieval Technique, Working Procedures in Microwave Histology and Microwave Processing Techniques for Microscopy & Histology.

Laboratory Protocols

An excellent resource with information about: cryostat sectioning, histologic fixatives, histologic stains, peroxidase techniques, treating slides for "stickiness", tissue processing and specimen collection (Veterinary Pathology, University of Bristol, UK).

Protocols On-Line - Immunology>Histological Techniques

StainsFile: A Resource for Histotechnologists

This site, developed by Bryan D. Llewellyn, is primarily about histologic staining techniques and dyes.

The Histology Page

Includes extracts from Woods and Ellis, "Laboratory Histopathology: A Complete Reference" (1994 Churchill Livingstone). Topics include: Cryotechniques, Fixation, Haematoxylin and Counterstains, Microtomy, Mountants, Resin Processing and Tissue Processing.

Techniques Manual

From the House Ear Institute (Paul Webster, Ph.D.).  This site has electron microscopy techniques as well as cytochemical and immuno staining techniques.

Tissue Preparation for Histology:

The WWW pages Use and Misuse of Formaldehyde Fixatives and Bouins Fixatives were developed for this site in response to some local concerns.

Immuno/Histo/Cyto-chemistry

Immunohistochemistry Home Page

A listing of immunohistochemistry resources on the Internet (Mahmood Aijazi, MD). Another resource is the Immunohistochemistry page of Roy Ellis, which includes suggestions for control tissues for different stains and a sample protocol that is in an ISO 9000 compliant format.

Journal of Histochemistry & Cytochemistry

"The official Journal of the Histochemical Society, publishes papers that report the development of new histochemical and cytochemical methods, significant modifications of existing techniques, or original research in which histochemical and cytochemical methods make a significant contribution." Many excellent full-text articles are available on-line.

Fluorescence Techniques

A web resource from this site with information about fluorescent dyes, antibodies and sample preparation.

Miscellaneous Protocol Resource Sites

BioVisa.net - Cell Biology>Microscopy & Histochemistry>Cell Labeling
BioVisa.net - Biotechnology > In-situ hybridization
Silverman Lab protocols (Children's Hospital, Boston)
NIEHS Laboratory of Experimental Pathology - Immunohistochemistry, In-Situ
ImmunoPortal.com - Immunohistochemistry & In-Situ Hybridization (Hogne Røed Nilsen)

Internet Resources

The HistoTech's Home Page

This list of Internet resources is intended to be used as a browser home page for Histotechnologists. (The National Society for Histotechnology)

HistoNet listserver

Scroll down this page to read the description of this email discussion forum for histotechnique (MicroWorld Resources). Visit this site to search the Archives of the HistoNet Listserver.

Histology Glossary

An on-line glossary of terms used to describe histologically prepared specimens (Eric Shelden).

Anatomy & Histology Center - Martindale's Health Science Guide

A collaborative effort of Jim Martindale and the University of California, Irvine (among others). This is an incredible compilation of links to histology & anatomy resources, as well as other biological links and some terrific general science reference material links.

WebPath

The Internet Pathology Laboratory for Medical Education (University of Utah, College of Medicine)

Images of Tissues prepared for Histology

LUMEN: Histology Index

This site is part of the Loyola University Medical Education Network (LUMEN).

Kansas "JayDoc" HistoWeb

From the Department of Anatomy & Cell Biology, University of Kansas.

University of Delaware - Mammalian Histology course

Includes images from histologic sections, electron microscopy, diagrams and 3D models.

Other Good General Histology Sites with Images:

University of Michigan - Histology course
University of Western Australia - Histology & Human Anatomy
University of Illinois - Internet Atlas of Histology
University of California at Davis - Human Microscopic Anatomy
Integrated Medical Curriculum - Histology (registration required)
University of Arkansas - Microanatomy Web Atlas
University of Southern California - General Histology, a digital atlas
University of Texas at Houston - Histology Review Carousels
University of Pennsylvania - Veterinary Histology
Indiana University - PERLjam Histology

Good sites for Images of specific tissues:

"The Whole Brain Atlas" - Harvard University
DERMIS - Dermatology On-line Atlas
Blood Morphology - University of Washington

Safety

Repetitive Strain Disorders

Some general background information on this problem (Leica Microsystems Nussloch GmbH).

The Vermont SIRI MSDS archive

If you can't locate the Material Safety Data Sheet that came with a particular product, this is a good place to start. Other MSDS resources include: MSDS Solutions.com and the Cornell University MSDS database.

Occupational Safety & Health Administration (OSHA, USA):

Laboratory Safety, Bloodborne Pathogens, Ionizing Radiation, Ergonomics